Protocols and Methods

Protocols.io

Versioned community protocols, including nuclear biology and chromatin workflows.

Open resource

Nature Protocols

Peer-reviewed methods articles and detailed workflows.

Open resource

Bio-protocol

Open protocols with reproducibility-focused detail.

Open resource

Hi-C 3.0

Improved genome-wide chromosome conformation capture protocol using dual cross-linking and dual restriction digestion (Lafontaine et al., 2021; Akgol Oksuz et al., 2021).

Open protocol Benchmark study

Micro-C

Micrococcal nuclease-based chromosome folding assays resolving nucleosome-scale chromatin architecture in mammalian cells (Hsieh et al., 2020; Krietenstein et al., 2020).

Hsieh et al. (2020) Krietenstein et al. (2020)

CUT&RUN

Antibody-targeted chromatin profiling with low background.

Open resource

CUT&TAG

Tagmentation-based chromatin profiling with low input requirements.

Open resource

SPRITE

Multi-way chromatin and RNA interaction mapping.

Open resource

TSA-seq

Genome proximity mapping to nuclear landmarks.

Open resource

DamID

DNA adenine methyltransferase identification protocol for mapping in vivo protein–DNA interactions and lamina-associated domains (Vogel, Peric-Hupkes & van Steensel, 2007).

Open resource

CiteAb

Citation and use evidence for commercial antibodies.

Open resource

Antibodypedia

Antibody validation data by application and target.

Open resource