Reverse-Engineering the Spliceosome
Biophysics of Cajal bodies and snRNP assembly pathways.
RNA Splicing
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FIB-SEM Studies
High-resolution focused ion beam scanning electron microscopy revealing nuclear ultrastructure and chromatin organization in unprecedented detail.
Data courtesy of Janelia Farms Research Campus
Macrophage Nucleus - PcG Subregions
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Technique: FIB-SEM multiview reconstruction
Sample: Macrophage nucleus showing Polycomb group (PcG) protein subregions
Resolution: Nanometer-scale 3D visualization
File Size: 381 MB
Featured Video
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PcG Subregion Analysis - View 3
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Technique: FIB-SEM serial sectioning
Focus: Alternative view of PcG subregion organization
Application: Chromatin territory mapping
File Size: 76.8 MB
High Resolution
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Shadow Volume Reconstruction
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Technique: Shadow volume visualization
Enhancement: 3D depth perception through shadow mapping
Purpose: Structural context and spatial relationships
File Size: 148.6 MB
3D Visualization
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HeLa Cell Analysis - Figure 1
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Cell Type: HeLa cervical cancer cell line
Application: Publication-quality figure generation
Features: Complete nuclear architecture visualization
File Size: 300.7 MB
Publication Quality
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Live-Cell Imaging
Dynamic visualization of nuclear processes in living cells, capturing real-time molecular movements and structural changes within the nuclear environment.
Nuclear Speckles Dynamics
Technical Details
Technique: Live-cell fluorescence microscopy
Marker: GFP-labeled nuclear speckle proteins
Cell Line: HeLa cervical cancer cells
Duration: Time-lapse sequence
Biological Significance
Nuclear speckles are dynamic subnuclear compartments enriched in pre-mRNA splicing factors.
This visualization captures their mobility and structural reorganization during cellular processes.
Critical for understanding RNA processing and gene expression regulation.
Live Imaging
GFP Fluorescence
View Nuclear Speckles Reviews
Live-Cell Archive
Original time-lapse and 3D imaging from the Hendzel lab, 2005–2009: chromatin, PML bodies, splicing factor compartments, and nuclear mobility in living mammalian cells. Short clips are re-encoded for the web from the original acquisitions.
For the lecture these movies come from, see Nanostructure of the Genome.
Chromatin dynamics in living cells
GFP-tagged nucleus, 3D time-lapse (HeLa)
Time-lapse of a HeLa nucleus with bright nuclear foci; the box marks the imaged volume. Replaces the previous speckle clip.
DsRed-H1 nucleus, orthogonal views
Deconvolved time-lapse of a DsRed-H1-labelled nucleus shown with XY, XZ and YZ projections (identified from the file name).
DsRed-H1 nucleus, orthogonal views (second cell)
A second DsRed-H1 time-lapse in the same display format, showing the nucleus in three planes.
Mouse cells: DIC and Hoechst
Mouse cells imaged by DIC (left) and Hoechst-stained chromatin (right).
Hoechst-stained nuclei with DIC context
Hoechst-stained nuclei (top) alongside the DIC view of the same field (bottom).
MO59J cell nucleus, live
Wide-field time-lapse of an MO59J cell nucleus with a 10 µm scale bar.
Nuclear bodies & splicing factor compartments
MCF7 cells: PML bodies
Two-colour time-lapse of MCF7 cell nuclei with PML-body labelling (from the file name; PML/DsRed).
PML bodies and SC35 speckles
Three-panel time-lapse comparing PML bodies, a nuclear overview, and SC35-labelled splicing factor compartments.
Three-colour nucleus (20 min series)
Merged and single-channel views of one nucleus with multiple nuclear-body markers, 10 µm scale bar.
3D view of SC35 compartments
Rotating 3D reconstruction of an elongated nucleus: DNA in blue, SC35-defined splicing factor compartments in green.
Splicing compartments in an Indian muntjac nucleus (3D)
3D rendering of an Indian muntjac nucleus with chromatin and SC35 compartments.
Mouse nucleus: 3D time series
Side-by-side 3D renderings of one mouse nucleus (DNA blue; nuclear bodies green) over time.
3D nuclear organization
Deconvolved DAPI nucleus: surface rendering
Surface-rendered 3D reconstruction of a DAPI-stained nucleus after deconvolution; segmented compartments coloured separately.
Tubulin and Hoechst
Cells with a cytoplasmic tubulin label (green) and Hoechst-stained nuclei (red), showing the nucleus in its cytoskeletal context.
Data Attribution
All FIB-SEM datasets presented in this gallery were generously provided by Janelia Farms Research Campus, a world-leading institution in biological imaging and neuroscience research. The Live-Cell Archive movies are original Hendzel lab acquisitions (2005–2009).
Advanced Imaging Facility
Open Science Initiative
Research Collaboration
Explore academic research video series
Find selected YouTube seminars, workshops, and research lectures on 4D genome organization, chromatin, nucleosomes, and epigenetics.
Each collection includes an organizer archive and notes on how its topics connect to nuclear biology.