Diffusion of 40 nm fluorescent spheres.
Mouse fibroblast nuclei were microinected with 40 nm fluorescent spheres. In some cases, cells were also transfected with GFP-SC35 and/or stained for DNA. Experiments were performed in the Michael J Hendzel Laboratory in the Department of Oncology at the University of Alberta.
Nanospheres Movie 1.
Nanospheres Movie 1.
Nanospheres Movie 2: DNA is green, 40 nm spheres are red.
Nanospheres Movie 2: DNA is green, 40 nm spheres are red.
Nanospheres Movie 3: DNA is red, 40 nm spheres are green.
Nanospheres Movie 3: DNA is red, 40 nm spheres are green.
Nanospheres Movie 4: DNA is red, 40 nm spheres are green.
Nanospheres Movie 4: DNA is red, 40 nm spheres are green.
Nanospheres Movie 5: DNA is green, 40 nm spheres are red.
Nanospheres Movie 5: DNA is green, 40 nm spheres are red.
Nanospheres Movie 6: DNA is blue, 40 nm spheres are red, and SC35-GFP is green.
Nanospheres Movie 6: DNA is blue, 40 nm spheres are red, and SC35-GFP is green.
Nanospheres Movie 7: DNA is blue, 40 nm spheres are red, and SC35-GFP is green.
Nanospheres Movie 7: DNA is blue, 40 nm spheres are red, and SC35-GFP is green.
Nanospheres Movie 8: DNA is blue, 40 nm spheres are red, and SC35-GFP is green.
Nanospheres Movie 8: DNA is blue, 40 nm spheres are red, and SC35-GFP is green.
Nanospheres Movie 9: DNA is green, 40 nm spheres are red.
Nanospheres Movie 9: DNA is green, 40 nm spheres are red.
Nanospheres Movie 10: Hoechst is green, Rhodamine dCTP labelled euchromatin is red. 40 nm spheres are also red.
Nanospheres Movie 10: Hoechst is green, Rhodamine dCTP labelled euchromatin is red. 40 nm spheres are also red.
Nanospheres Movie 11: DNA is blue, 40 nm spheres are red, SC35-GFP is green.
Nanospheres Movie 11: DNA is blue, 40 nm spheres are red, SC35-GFP is green.
Nanospheres Movie 12: 40 nm spheres.
Nanospheres Movie 12: 40 nm spheres.
Nanospheres Movie 13: Same as Nanospheres Movie 8 with the colours changed such that the beads are green and the DNA is red.
Nanospheres Movie 13: Same as Nanospheres Movie 8 with the colours changed such that the beads are green and the DNA is red.
Nanospheres Movie 14: Same as Nanospheres Movie 8 but filtered using an edge filter to define the boundaries of structures.
Nanospheres Movie 14: Same as Nanospheres Movie 8 but filtered using an edge filter to define the boundaries of structures.
Nanospheres Movie 15: 40 nm spheres are red, DNA is green.
Nanospheres Movie 15: 40 nm spheres are red, DNA is green.
Nanospheres Movie 16: Same as movie 8 and 14.
Nanospheres Movie 16: Same as movie 8 and 14.
Nanospheres Movie 17: 40 nm spheres
Nanospheres Movie 17: 40 nm spheres
Nanospheres Movie 18: 40 nm spheres.
Nanospheres Movie 18: 40 nm spheres.
Nanospheres Movie 19: 40 nm spheres are green, DNA is red.
Nanospheres Movie 19: 40 nm spheres are green, DNA is red.
Nanospheres Movie 20: High speed images. Elapsed time is in min:sec:1/100 sec.
Nanospheres Movie 20: High speed images. Elapsed time is in min:sec:1/100 sec.
Nanospheres Movie 21: DNA is green, 40 nm spheres is red. The cytoplasmic signal is autofluorescence.
Nanospheres Movie 21: DNA is green, 40 nm spheres is red. The cytoplasmic signal is autofluorescence.
Nanospheres Movie 22: 40 nm spheres.
Nanospheres Movie 22: 40 nm spheres.
Nanospheres Movie 23: High speed images. Elapsed time is seconds and 1/10 seconds.
Nanospheres Movie 23: High speed images. Elapsed time is seconds and 1/10 seconds.